The A12 is capable of recording pretty nice looking 1080p/30 fps videos in bright light, but stabilization isn’t too effective and limited dynamic range means high-contrast conditions are best avoided. Importantly, these data present a mechanistic basis for the requirement of A12.2 in Pol I termination. Samsung Galaxy A12, crop: noise and low level of detail good target exposure but limited dynamic range. In addition, the salt concentration dependence of Pol I EC dissociation kinetics suggests that A12.2 alters electrostatic interactions within the EC. Using, to our knowledge, a novel assay to measure EC dissociation kinetics, we have determined that A12.2 is an intrinsic destabilizer of the Pol I EC. Given that termination involves destabilization of the elongation complex (EC), we tested whether A12.2 influences stability of the Pol I EC. Specifically, it appears that both A12.2 and C11 are required for efficient termination of transcription by Pols I and III. Although it is not yet clear why these polymerases have incorporated their cleavage factors as subunits, a picture is emerging that identifies roles for these subunits beyond providing nucleolytic activity.
Whereas all known cellular Pols possess cognate protein factors that stimulate cleavage of the nascent RNA, Pols I and III have incorporated their cleavage factors as bona fide subunits. Interesting examples of structural divergence are the A12.2 and C11 subunits of Pols I and III, respectively. Despite sharing a highly conserved core architecture with their prokaryotic counterparts, eukaryotic multisubunit RNA polymerases (Pols) have undergone structural divergence and biological specialization.
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